Virus-Host Interactions: Methods and Protocols covers various aspects of virological research, such as biochemical approaches, including molecular interactions and regulatory mechanisms on the protein as well as the RNA level with a strong focus on the manifold possibilities to study protein-protein interactions, as well as cell biological and immunological methodologies. Viruses represent a reduced form of life that depends on host cells for propagation. To this end, viruses approach and penetrate cells and usurp cellular machineries for their own benefit. Recent technological improvements have enabled the systematic analysis of the virus-host interplay be it on the genomic, the transcriptomic, or proteomic level. In parallel, bioinformatic tools have emerged in support of the large datasets generated by these high-throughput approaches. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Virus-Host Interactions: Methods and Protocols will prove invaluable to professionals and novices with its well-honed methodologies and protocols.

Inhaltsangabe1. A High-Throughput Yeast Two Hybrid Protocol to Determine Virus-Host Protein Interactions            Hannah Striebinger, Manfred Kögl, and Susanne M. Bailer 2. Analysis of Protein-Protein Interactions Using LUMIER AssaySonja Blasche and Manfred Koegl 3. Detection of Protein Interactions During Virus Infection by Bimolecular Fluorescence Complementation            Stefan Becker and Jens von Einem 4. Discovery of Host-Viral Protein Complexes During InfectionDaniell L. Rowles, Scott S. Terhune, and Ileana M. Cristea 5. Screening for Host Proteins with Pro- and Antiviral Activity Using High-Throughput RNAi            Samantha J. Griffiths 6. High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay by Metabolic Labelling of Newly Transcribed RNA using 4-thiouridine            Lars Dölken 7. Isolation and Characterization of Pathogen-Bearing Endosomes Enables Identification of New Cellular Cofactors and Analysis of Endosomal Escape            Konstanze D. Scheffer, Ruth Popa-Wagner and Luise Florin 8. Computational Analysis of Virus-Host Interactomes            Caroline C. Friedel 9. Interspecies Heterokaryon Assay to Characterize the Nucleocytoplasmic Shuttling of Herpesviral Proteins            Shuai Wang, Kezheng Wang, and Chunfu Zheng 10. Detection of Integrated Herpesvirus Genomes by Fluorescent in situ Hybridization (FISH)            Benedikt Kaufer 11. Fast Generation of Stable Cell Lines Expressing Fluorescent Marker Molecules to Study Pathogen Induced Processes            Jens Bernhard Bosse, Jessica Ragues, and Harald Wodrich 12. Determination of HSV-1 Infectivity Using a Luciferase Reporter Cell Line            Diana Lieber and Susanne M. Bailer 13. Generation of a Cell Line for Constitutive Expression of Host or Viral miRNAs            Diana Lieber 14. Applications for a Dual Fluorescent Human Cytomegalovirus in the Analysis of Viral Entry                Kerstin Laib Sampaio, Gerhard Jahn, and Christian Sinzger 15. High Temporal Resolution Imaging Reveals Endosomal Membrane Penetration and Escape of Adenoviruses in Real-Time            Ruben Martinez, Andrew M. Burrage, Christopher M. Wiethoff, and Harald Wodrich 16. Three-Dimensional Visualization of Virus Infected Cells by Serial Sectioning - An Electron Microscopic Study Using Resin Embedded Cells            Martin Schauflinger, Clarissa Villinger, and Paul Walther 17. 3D Tissue Model for Herpes Simplex Virus-1 Infections            Ina Hogk, Steffen Rupp, and Anke Burger-Kentischer 18. In vivo Visualization of Encephalitic Lesions in Herpes Simplex Virus Type 1 (HSV-1) Infected Mice by Magnetic Resonance Imaging (MRI)            Wali Hafezi and Verena Hoerr 19. Detection of Antigen-Specific T Cells Based on Intracellular Cytokine Staining Using Flow-Cytometry            Tina Schmidt and Martina Sester 20. Functional NK Cell Cytotoxicity Assays Against Virus Infected Cells            Rebecca J. Aicheler and Richard J. Stanton  21. Assessment of Natural Killer Cell Responses to Human Cytomegalovirus Infected Macrophages            Zeguang Wu, Giada Frascaroli, and Thomas Mertens 22. Analysis of Cell Migration During HCMV Infection            Stefania Varani and Giada Frascaroli 23. Gene Targeting in Mice: A Review            Hicham Bouabe and Klaus Okkenhaug 24. A Protocol for Construction of Gene Targeting Vectors and Generation of Homologous Recombinant ES Cells            Hicham Bouabe and Klaus Okkenhaug 25. Measurement of Mouse Cytomegalovir