Blood science has become a cornerstone of multiple disciplines, including clinical chemistry, disease diagnosis, and therapeutic monitoring.  Over the past decade, we have witnessed the advent of increasingly powerful proteomics technologies that allow greater fundamental insights into the blood proteome.  These technological improvements have, in part, fuelled the quest for the discovery of novel blood-based biomarkers of disease. Serum/Plasma Proteomics: Methods and Protocols is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, this detailed volume covers fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays,  proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, and finally data management, statistical design, and bioinformatic challenges. This book, contributed to by leading experts in the field, provides a valuable foundation for the development and application of blood-based proteomics. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Serum/Plasma Proteomics: Methods and Protocols, with its well-honed methodologies, seeks to serve both professionals and investigators new to the field in an effort to further our knowledge of this fundamental science.

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Humana Press in Springer Science + Business Media
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Part I. Fractionation Strategies for In-depth Blood Proteome Analysis   1. Plasma Biomarker Discovery using 3-D Protein Profiling Coupled with Label-free Quantitation             Lynn A. Beer, Hsin-Yao Tang, Kurt T. Barnhart, and David W. Speicher   2. Intact Protein Separation by 1 and 2-dimension Liquid Chromatography for Comparative Proteomic Separation of Partitioned Serum or Plasma             Simon Sheng, Helena Skalnikova, Andrew Meng, Qin Fu, Allen Everett, and Jennifer E. Van Eyk   3. In-depth Analysis of a Plasma or Serum Proteome using a 4-D Protein Profiling Method             Hsin-Yao Tang, Huan Wang, Lynn A. Beer, and David W. Speicher   4. Intact-protein Analysis System (IPAS) for Discovery of Serum-based Disease Biomarkers             Hong Wang and Samir Hanash   5. Model-based Discovery of Circulating Biomarkers             Maryann S. Vogelsang and Parag Mallick   6. Low-molecular Weight Plasma Proteome Analysis using Centrifugal Ultrafiltration             David W. Greening and Richard J. Simpson   7. High-throughput Analysis of Glycoproteins from Plasma             Yan Li and Hui Zhang   Part II. Blood Collection and Handling Strategies   8. Minimizing Pre-analytical Variation of Plasma Samples by Proper Blood Collection and Handling             Jizu Yi, David Craft, and Craig A. Gelfand   9. Collection and Handling of Blood Specimens for Peptidomics             Harald Tammen and Rüdiger Hess   10. Investigation of Peptide Biomarker Stability in Plasma Samples using Time-course MS Analysis             Jizu Yi, Zhaoxia Liu, Craig A. Gelfand, and David Craft   Part III. Methods for Quantitative Assaying of Blood-Based Biomarkers   11. Biomarker Validation in Blood Specimens by Selected Reaction Monitoring Mass Spectrometry of N-glycosites             Reto Ossola, Ralph Schiess, Paola Picotti, Oliver Rinner, Lukas Reiter, and Ruedi Aebersold   12. A Fluorescent Microsphere-based Method for Assay of Multiple Analytes in Plasma    Oliver K. Bernhard, Rommel A. Mathias, Thomas W. Barnes, and Richard J. Simpson   13. Immuno-mass Spectrometry: Quantification of Low Abundance Proteins in Biological Fluids             Vathany Kulasingam, Christopher R. Smith, Ihor Batruch, and Eleftherios P. Diamandis   14. Qualitative and Quantitative Analysis of Glycated Proteins in Human Plasma by Glucose Isotopic Labeling with 13C6-reducing Sugars             Feliciano Priego-Capote, Maria Ramírez-Boo, Denis Hochstrasser, and Jean-Charles Sanchez   Part IV. Proteome Analysis of Blood Cell Components, Vesicles, and Blood-related Fluids   15. Exosome Isolation for Proteomic Analyses and RNA Profiling             Douglas D. Taylor, Wolfgang Zacharias, and Cicek Gercel-Taylor   16. Extraction and Proteome Analysis of Liver Tissue Interstitial Fluid             Wei Sun, Ying Jiang, and Fuchu He   17. A Protocol for the Preparation of Cryoprecipitate and Cryo-depleted Plasma             Rosemary L. Sparrow, David W. Greening, and Richard J. Simpson   18. Preparation of Platelet Concentrates             David W. Greening, Richard J. Simpson, and Rosemary L. Sparrow   19. Phosphoproteome Analysis of the Platelet Plasma Membrane             Thomas Premsler, Urs Lewandrowski, Albert Sickmann, and Rene Peiman Zahedi   Part V. Bioinformatic Analysis of Blood Proteins and Peptides   20. Statistical Design and Analysis of Label-free LC-MS Proteomic Experiments: A Case Study of Coronary Artery Disease             Timothy Clough, Siegmund Braun, Vladimir Fokin, Ilka Ott, Susanne Ragg, Gunther Schadow, and Olga Vitek   21. Data Management in Mass Spectrometry-based Proteomics             Lennart Martens   22. Bioinformatics Challenges in the Proteomic Analysis of Human Plasma             Joseph M. Foster and Lennart M ...