A single volume collection that surveys the exciting field of plant-made pharmaceuticals and industrial proteins

This comprehensive book communicates the recent advances and exciting potential for the expanding area of plant biotechnology and is divided into six sections. The first three sections look at the current status of the field, and advances in plant platforms and strategies for improving yields, downstream processing, and controlling post-translational modifications of plant-made recombinant proteins. Section four reviews high-value industrial and pharmacological proteins that are successfully being produced in established and emerging plant platforms. The fifth section looks at regulatory challenges facing the expansion of the field. The final section turns its focus toward small molecule therapeutics, drug screening, plant specialized metabolites, and plants as model organisms to study human disease processes.

Molecular Pharming: Applications, Challenges and Emerging Areas offers in-depth coverage of molecular biology of plant expression systems and manipulation of glycosylation processes in plants; plant platforms, subcellular targeting, recovery, and downstream processing; plant-derived protein pharmaceuticals and case studies; regulatory issues; and emerging areas. It is a valuable resource for researchers that are in the field of plant molecular pharming, as well as for those conducting basic research in gene expression, protein quality control, and other subjects relevant to molecular and cellular biology.

Providing wide-ranging and unique coverage,Molecular Pharming: Applications, Challenges and Emerging Areas will be of great interest to the plant science, plant biotechnology, protein science, and pharmacological communities.

Allison R. Kermode is Professor and Michael Smith Senior Scholar at Simon Fraser University. Her work on plant-derived human proteins was recently featured inNature magazine. Dr. Kermode is also the editor ofSeed Dormancy: Methods and Protocols.

Liwen Jiang is Choh-Ming Li Professor of Life Sciences at the Chinese University of Hong Kong. He is a leader in research on protein targeting and trafficking in plant cells, plant organelle biogenesis and function, and the application of these processes to plant biotechnology.

List of Contributors ix

Preface xiii

Part One The Molecular Farming/Pharming Landscape 1

1 Current Status and Perspectives of the Molecular Farming Landscape 3
Holger Spiegel, Eva Stöger, Richard M. Twyman, and Johannes F. Buyel

Part Two Molecular Biology of Plant Expression Systems and Manipulation of Glycosylation Processes in Plants 25

2 Synthetic Transcription ActivatorLike EffectorActivated Promoters for Coordinated Orthogonal Gene Expression in Plants: Applications for Regulatory Circuit and Metabolic Engineering 27
Tom Schreiber and Alain Tissier

3 Contemporary and Emerging Technologies for Precise Nglycan Analyses 43
Iain B.H. Wilson, Katharina Paschinger, Jorick Vanbeselaere, and Chunsheng Jin

4 Production of Functionally Active Recombinant Proteins in Plants: Manipulating N and Oglycosylation 67
Alexandra Castilho and Richard Strasser

Part Three Plant Platforms, Subcellular Targeting, Recovery, and Downstream Processing 91

5 Seeds as Bioreactors 93
Jinbo Shen, Xiangfeng Wang, and Liwen Jiang

6 Strategies to Increase Expression and Accumulation of Recombinant Proteins 119
Reza Saberianfar and Rima Menassa

7 The Impact of Six Critical Impurities on Recombinant Protein Recovery and Purification from Plant Hosts 137
Chelsea Dixon, Lisa R. Wilken, Susan L. Woodard, and Georgia O.F. Barros

8 Plant Recombinant Lysosomal Enzymes as Replacement Therapeutics for Lysosomal Storage Diseases: Unique Processing for Lysosomal Delivery and Efficacy 181
Allison R. Kermode, Grant McNair, and Owen Pierce

Part Four PlantDerived Protein Pharmaceuticals and Case Studies 217

9 PlantProduced Antibodies and PostTranslational Modification 219
Andreas Loos and Herta Steinkellner

10 Molecular Pharming: PlantMade Vaccines 231
Qiang Chen, Matthew Dent, and Hugh Mason

11 Transgenic Rice for the Production of Recombinant Pharmaceutical Proteins: A Case Study of Human Serum Albumin 275
Daichang Yang, Jiquan Ou, Jingni Shi, Zhibin Guo, Bo Shi, and Naghmeh Abiri

12 Enzymes for Industrial and Pharmaceutical Applications  From Individual to Population Level Impact 309
Elizabeth E. Hood and Carole L. Cramer

Part Five Regulatory Issues 327

13 Biosafety, Risk Assessment, and Regulation of Molecular Farming 329
Penny A.C. Hundleby (nee Sparrow), Markus Sack, and Richard M. Twyman

Part Six Emerging Areas: Plant Specialized Metabolites and Small Molecule Drugs 353

14 Harnessing Plant Trichome Biochemistry for the Production of Useful Compounds 355
Alain Tissier

15 Reconstitution of Medicinally Important Plant Natural Products in Microorganisms 383
Ozkan Fidan and Jixun Zhan

16 Screening of Epidermal Growth Factor Receptor Inhibitors in Natural Products Derived From Extracts of Traditional Chinese Medicines 417
Li Feng and Jingwu Kang

17 Target-Directed Evolution of Mutant Transgenic Plant Cells as a Novel Source of Drugs 435
John Littleton, Dustin Brown, Deane Falcone, Gregory Gerhardt, Samir Gunjan, Dennis T. Rogers, and Jatinder Sambi

18 Plant Thermotolerance Proteins, Misfolded Proteins, and Neurodegenerative Diseases 457
Indranil Basak and Simon G. Møller

Index 475